Rock Inhibitor Stem Cell Technologies

H1 human embryonic stem hes cells were seeded at clonal density 100 cells well 25 cells cm 2 in mtesr 1 supplemented with a rock inhibitor or b cloner on vitronectin xf cell culture matrix.

Rock inhibitor stem cell technologies.

Critical media component in conditional reprogramming of cells crc culture technology 2. For rock inhibitor treatment y 27632 calbiochem. Y 27632 is a cell permeable highly potent and selective inhibitor of rho associated coiled coil containing protein kinase rock. Shown are representative images of alkaline phosphatase stained colonies at day 7 in individual wells of a 12 well plate.

Y 27632 inhibits both rock1 ki 220 nm and rock2 ki 300 nm by competing with atp for binding to the catalytic site davies et al. Thiazovivin is effective at 5 fold lower concentrations than another common rock inhibitor y 27632 catalog 72302. 2014 reversal of diabetes with insulin producing cells derived in vitro from human pluripotent stem cells. Rock inhibitor y27632 atcc acs 3030 to atcc valued customers.

Enhanced survival and maintenance of pluripotency of human embryonic stem cells hesc and human induced pluripotent stem cells hipsc 1. Li x et al. Maintenance and self renewal promotes survival of human embryonic stem es cells during dissociation by stabilizing e cadherin and improves cell attachment xu et al.